BRAF - DNA Mutation RT - PCR
BRAF is a gene that synthesizes proteins involved in cell growth. Due to any mutation in the BARF gene, cell growth can’t be controlled i.e hence its growth results in uncontrolled proliferation (unstoppable), thus it involves in the formation of Cancerous growth. DNA test for BARF gene by RT-PCR detects the mutation on BARF gene cells usually seen in cases such as in melanoma and other BARF cancer-related cases in patients.
BRAF:
Why BRAF Test?
CLINICAL INFORMATION
BRAF oncogenes codes for a protein that is important in the cell growth (BRAF protein helps control cell growth) of some cancers (leading to uncontrolled cell growth – MAPK pathway, that can lead to cancer like Lynch syndrome –HNPCC; in patients with microsatellite unstable cancer) such as metastatic melanoma, lung cancer, colon cancer, skin cancer, thyroid, histiocytic tumors, certain brain tumors, etc. if BRAF is tested positive, the presence of BRAF mutation may help guide treatment (such as medications that target BRAF mutations like panitumumab or cetuximab used in monoclonal antibody treatment for metastatic colorectal cancer and/or small molecule drugs that block the growth and spread of cancer progression), estimate prognosis. BRAF test is done to screen for the presence of BRAF mutation (amino acid valine to glutamic acid substitution at position 600, which causes uncontrolled signaling of the MAPK pathway leading to excessive cell growth and proliferation) in a tumor (cancer screening). This test can be done by different techniques, such as immunohistochemistry, or comprehensive genetic profiling, blood test (liquid biopsy) cell-free DNA – cfCNA in peripheral blood for the presence of BRAF V600E or V600K alterations in patients with melanoma and/or tumor biopsy sample (FNAC) testing by blotting techniques – PCR based genetic testing (extraction of genomic DNA and/or PER amplification of target DNA). Hence BRAF gene testing has emerged as an important tool for diagnosis, prognosis, treatment, and predicting patient outcome in response to targeted therapy for multiple cancer types.
General Instructions:
Sample Requirement: Specimen - Blood sample collected from the vein. Test Preparation: None.
NOTE - Sample for specimen collections may vary based on the patient’s condition/cases according to the patient’s presenting complaints/signs or symptoms:
SPECIMEN REQUIREMENT (Special or Rare Cases) - As instructed and guided by Physician / Clinician / Pathologist / as per Laboratory’s requirements, according to procedures and protocols.
Sample Requirement: Tissue from the Tumor
Test Preparation: None
This Multi-Specialty Clinical Referral Laboratory RT DIAGNOSTICS provides precise and accurate tests with an extensive range of testing services to the medical centers to help in the diagnosis and identification of pathology in the test specimens for infectious diseases and also to evaluate the function of organ systems of the patient. It prevents further complications and helps to stabilize and restore health to near normalcy at the earliest without delay.
